Cordyceps militaris Mushroom Cultivation Method
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Cordyceps militaris Mushroom Cultivation Method |
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Cordyceps militaris Mushroom Cultivation Method
Scientific name: Cordyceps spp.
Family: Cordycipitaceae
Common name: Scarlet caterpillar club, Caterpillar killer
General appearance: It forms 1 - 8 cm high, club shaped and orange/ red fruiting bodies, which grow out of dead underground pupae. The upper portion of the fungus is orange and pimply, the lower portion smooth and orange to pale orange, often curved; narrowing at the base.
Benefits of Cordyceps mushroom:
Produce cordycepin (3’-deoxyadenosine)
Anti-aging property
May boost exercise performance
Potential anti-tumor effects
May help to manage type 2 diabetes
Possible benefits for heart health
Help to fight inflammation
Growth parameter:
Substrate: Supplemented black rice is best
Temperature: 16-240 C
Humidity: 70-80%
Cultivation method
This mushroom is cultivated under lab conditions where facilities for control of temperature, humidity and filtered fresh air are available. Cultivation is done in jars kept on racks just like we keep bags or bottles in spawn lab.
First step in preparation of the substrate is the preparation of nutrient solution. For this we add the following in 1000 ml distilled water.
Glucose- 20 g, Peptone - 5 g, Yeast extract - 3 g, KH2PO4 - 2 g, MgSO4 - 0.5 g, Tri Ammonium Citrate (TCA) - 1 g, Vit B1 - 50 mg, Vit B12 - 10 mg, Multivitamin - 10 mg
Second step is the supplementation of brown rice with above nutrient medium. Brown rice is soaked in water for 30 minutes, cleaned and washed thoroughly.
Thereafter, it is kept on a sieve or cotton cloth and allowed to dry for 30 minutes (various workers have used substrates other than rice also for the cultivation of this mushroom).
Take 20-25 g rice per jar, add about 40 ml nutrient solution to it and cover the jar with autoclavable cap or polypropylene bag and autoclave at 15 psi for 40-50 minutes.
A small hole is made in the cap and it is plugged with cotton.
Cool the jars and add 5-10 ml liquid spawn. Whole operation is done in Laminar Flow cabinet to ensure that there is no contamination. Jars are moved left to right to spread the liquid medium over the solid medium.
After inoculation with liquid spawn the jars are kept in dark for 8-10 days at 20-22°C, RH 65-70%.
After complete colonization of the substrate, the jars are kept in light for a week when the colour of mycelium turns orange.
Provide 12 hours light daily.
It may take 7 days for yellowing, another 2 weeks for bubble and then pinheads formation.
When distinct pinheads are formed, we can reduce the light to 8-12 hours and it may take another 5-6 week till mushrooms attain harvestable size of 4-6 cm.
Total time required for one crop from inoculation to harvest can be up to 3 months.
When head of mushroom turns club shaped, it is ready for harvest. The mushrooms are plucked out of jars and dried.
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