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| Name | Single spore encapsulation using droplet-based microfluidics, by Sehrish Iftikhar |
| Video Uploader | Video From Elveflow Microfluidics |
| Upload Date | This Video Uploaded At 07-08-2020 09:15:10 |
| Video Discription | To learn more: https://www.elveflow.com/microfluidic-applications/setup-droplet-and-digital-microfluidics/how-to-perform-single-spore-encapsulation/ Highly monodispersed droplets can be easily generated to encapsulate single spore of fungus using a microfluidic droplet generation system. In this work, several experimental conditions have been tested to achieve the encapsulation of single spore of fungi within highly monodispersed droplets. This technique can be adapted to encapsulate different type of fungus, with different spore size (ex: Alternaria alternata, A. tenuissima, Curvularia spp. Fusarium spp. etc.) or even cells. We will first start by preparing a spore suspension solution at a precise concentration according to the Poisson Law distribution. Then microdroplets of this solution will be generated using a microfluidic system. There are various advantages to single spore encapsulation, including protection against coarse external environment, physical and chemical isolation of spores, less chances of contamination against foreign organisms, enhanced isolation and fast and efficient reagent mixing. To go further: - Microfluidics for fungus identification and research: A review: https://www.elveflow.com/microfluidic-reviews/microfluidics-for-cell-biology/microfluidic-for-fungus-identification-and-research-a-review/ - The MAHT-FunSST project: Microfluidic Lab-on-chip for High-Throughput Fungicide Screening https://www.elveflow.com/group/microfluidic-research-projects/microfluidic-lab-on-chip-for-high-throughput-fungicide-screening-maht-funsst/ |
| Category | Science & Technology |
| Tags | Microfluidics for fungus identification and research | Elveflow | fungus | fungus identification | elvesys | Single spore encapsulation | droplet-based microfluidics | microfluidics | highly monodispersed droplets |
