Hellow guys, Welcome to my website, and you are watching Prime Editing - In almost less than a minute. and this vIdeo is uploaded by BoredButResourceful at 2020-01-17T07:22:36-08:00. We are pramote this video only for entertainment and educational perpose only. So, I hop you like our website.
| Name | Prime Editing - In almost less than a minute |
| Video Uploader | Video From BoredButResourceful |
| Upload Date | This Video Uploaded At 17-01-2020 15:22:36 |
| Video Discription | Prime Editing is a new version of the popular CRISPR-Cas9 gene editing methodology. As far as I can gather from their paper, this is how it works. Word list: - PAM: Protospacer Adjacent Motif, used in CRISPR for targeting. - pegRNA: prime editing guide RNA. - Prime Editor: a modified Cas9 which only has nickase activity (not nuclease, so no double stranded breaks) and an added reverse transcriptase domain (for converting RNA to DNA). - Nick: Single stranded break in the DNA. - Flap: A strand of DNA complementary to the unedited strand. The video is based on a paper from these fellas: Anzalone, A.V., Randolph, P.B., Davis, J.R. et al. Search-and-replace genome editing without double-strand breaks or donor DNA. Nature 576, 149–157 (2019) doi:10.1038/s41586-019-1711-4 |
| Category | Education |
| Tags | pam site | crispr | crispr-cas9 | cas9 | gene editing | genomics | genomic | prime editing | genomic editing | gmo | gene modification | educational | quick | fast | editing | pegRNA | prime editor | grna | guide rna | dna | how it works | tutorial | explained | prime editing explained | how prime editing works | what is prime editing | new version of crispr | animation | powerpoint | boredbutresourceful | how does prime editing work | genetics | edit | rna | target | double strand | markerless | marker-less | pam | hairpin | modification |
