How to dissociate FFPE tissue
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How to dissociate FFPE tissue |
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Video From Miltenyi Biotec |
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This Video Uploaded At 11-04-2019 13:43:31 |
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Watch how you can easily dissociate FFPE tissue in some easy steps using the gentleMACS™ Octo Dissociator with Heaters and the FFPE Tissue Dissociation Kit. This method has been optimized for the subsequent isolation of tumor and non-tumor cells from human carcinomas.
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https://www.miltenyibiotec.com/DE-en/products/macs-sample-preparation/tissue-dissociation-kits/ffpe-tissue-dissociation-kit.html#130-118-052
The FFPE Tissue Dissociation Kit together with the gentleMACS™ Octo Dissociator with Heaters, allows a gentle and effective generation of single-cell suspensions from human formalin-fixed paraffin-embedded (FFPE) carcinoma sections. It preserves the important cell epitopes cytokeratin for cell analysis of carcinoma cells and vimentin for non-carcinoma cells. The isolated target cells are ready for molecular downstream applications such as mutation analysis by next-generation sequencing or ploidy analysis by flow cytometry.
Miltenyi Biotec is a global provider of products and services that advance biomedical research and cellular therapy. Our innovative tools support research at every level, from basic research to translational research to clinical application. This integrated portfolio enables scientists and clinicians to obtain, analyze, and utilize the cell. Our technologies cover techniques of sample preparation, cell isolation, cell sorting, flow cytometry, cell culture, molecular analysis, and preclinical imaging. Our more than 25 years of expertise spans research areas including immunology, stem cell biology, neuroscience, and cancer, and clinical research areas like hematology, graft engineering, and apheresis. In our commitment to the scientific community, we also offer comprehensive scientific support, consultation, and expert training. Today, Miltenyi Biotec has more than 2,000 employees in 25 countries – all dedicated to helping researchers and clinicians around the world make a greater impact on science and health. |
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Science & Technology |
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FFPE tissue | Tumor cell separation | Tumor cell isolation | FFPE sample preparation | formalin-fixed paraffin-embedded | Carcinoma sample | carcinoma section | gentleMACS™ Octo Dissociator | gentleMACS™ Technology | How to dissociate FFPE tissue |
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